Multiplex PCR protocol
Multiplex PCR protocol
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Categories: pcr, PCR protocols, Protocols, Real Time PCR Tags: multiplex, Multiplex PCR protocol, pcr, PROTOCOL
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Essential Bioinformatics Resources for Designing PCR Primers
| Essential Bioinformatics Resources for Designing PCR Primers – USC … |
| PAGE=Nucleotides&PROGRAM=blastn&MEGABLAST=on&BLAST_PROGRAMS=megaBlast&PAGE_TYPE= BlastSearch&SHOW_DEFAULTS=on.Resources for PCR Primer Specificity Analysis
AutoPrime – Primers for expressed sequences. AutoPrime allows the effortless design of primers for Real-Time PCR. It employs the Primer3 software and sequence information from the Ensembl database to automate the process to the highest degree.
http://www.autoprime.de/ CODEHOP: COnsensus-DEgenerate Hybrid Oligonucleotide Primers.PCR primers designed from protein multiple sequence alignments. The program is intended for cases where the protein sequences are distant from each other and degenerate primers are needed.
http://blocks.fhcrc.org/blocks/codehop.html GeneFisher: Interactive Primer Design
http://bibiserv.techfak.uni-bielefeld.de/genefisher/ Melting – this program computes, for a nucleic acid duplex, the enthalpy, the entropy and the melting temperature of the helix-coil transitions.
http://www.ebi.ac.uk/~lenov/meltinghome.html OLIGO 6, Primer Analysis Software for Mac and Windows.
http://www.medprobe.com OLIGO 6, Primer Analysis Software for Mac and Windows.
http://www.oligo.net Oligo Calculator
http://www.microbiology.adelaide.edu.au/learn/oligcalc.htm PCR primer selection program based on xprimer.
http://alces.med.umn.edu/webprimers.html Primer Designer 4 for Windows 95 — Software to design, analyze, mutate primers.
http://www.scied.com/ses_pd4.htm Primer Premier: Comprehensive primer design for Windows and Power Macintosh.
http://www.PremierBiosoft.com/ Primer3 – PCR primers from nucleotide sequence
http://bioinformatics.weizmann.ac.il/cgi-bin/primer/primer3.cgi Primerfinder – A tool to design oligonucleotides suitable for PCR within any sequence you specify. Primers are designed to meet your specific needs based on the information entered on the search page.
http://eatworms.swmed.edu/~tim/primerfinder/ Prodoric – prokaryotic database of gene-regulation and molecular networks.
http://www.prodoric.de Web Primer: DNA and Purpose Entry
http://genome-www2.stanford.edu/cgi-bin/SGD/web-primer You can easily design primers for PCR and DNA sequencing using our free Web DoPrimer software ! The sequence information you need can be retrieved on-the-fly from the EMBL sequence database.
http://doprimer.interactiva.de/ |