Carbonate Bicarbonate Buffer pH range 9.2 to 10.6

Carbonate Bicarbonate Buffer pH range 9.2 to 10.6

(a) 0.1 M Sodium carbonate (anhydrous), 10.6 g/l (M.W.: 106.0)

(b) 0.1 M Sodium bicarbonate, 8.4 g/l (M.W.: 84.0)

Mix sodium carbonate and sodium bicarbonate solutions in the proportions indicated

and adjust the final volume to 200 ml with deionized water. Adjust the final pH using

a sensitive pH meter.

ml of Sodium carbonate 4.0 9.5 16.0 22.0 27.5 33.0 38.5 42.5

ml of Sodium bicarbonate 46.0 40.5 34.0 28.0 22.5 17.0 11.5 7.5

pH 9.2 9.4 9.6 9.8 10.0 10.2 10.4 10.6pH 7.2 7.4 7.6 7.8 8.2 8.6 9.0

source: https://www.med.unc.edu/pharm/sondeklab/files/resource-files/protein-purification-handbooks/buffers_calbiochem.pdf