Protocols for Buffers


Buffer Formula (required precision; 2 %)

Always to try to prepare buffer solution at the temperature and concentration before planing to use during the experiment. Stock solutions are acceptable as long as pH adjustment made after temperature and concentration adjustment. Also good buffers, e.g., Tris and Phosphate, are stable for a long time period.

10X TE (10:2)

1X 1L 10X

10mM Tris-HCl 8.9 g Tris-HCl

5.3 g Tris-Base

2 mM Na3EDTA 7.4 g

pH 8 at 25 C. Mix and store at 4 C. Periodically dump the 1X TE and make up the fresh 1X TE from 10X stock.

10X TE Buffer Preparation .

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