Method for Denaturing Gradient Gel Electrophoresis – DGGE

Method: Denaturing Gradient Gel Electrophoresis (DGGE)

The purpose of the DGGE is  Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially the fragments move according to molecular weight, but as they progress into higher denaturing conditions, each (depending on its sequence composition) reaches a point where the DNA begins to melt. The partial melting severely retards the progress of the molecule in the gel, and a mobility shift is observed. It is the mobility shift which can differ for slightly different sequences (depending on the sequence, as little as a single bp change can cause a mobility shift). Alleles are detected by differences in mobility.


DGGE blotting,

DGGE- finding polymorphisms with PCR amplified DNA,

and Special Equipment which is needed in setting DGGE can be found in Protocol for DGGE

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