Essential Bioinformatics Resources for Designing PCR Primers

Essential Bioinformatics Resources for Designing PCR Primers – USC …
PAGE=Nucleotides&PROGRAM=blastn&MEGABLAST=on&BLAST_PROGRAMS=megaBlast&PAGE_TYPE= BlastSearch&SHOW_DEFAULTS=on.Resources for PCR Primer Specificity Analysis

AutoPrime – Primers for expressed sequences. AutoPrime allows the effortless design of primers for Real-Time PCR. It employs the Primer3 software and sequence information from the Ensembl database to automate the process to the highest degree.
CODEHOP: COnsensus-DEgenerate Hybrid Oligonucleotide Primers.PCR primers designed from protein multiple sequence alignments. The program is intended for cases where the protein sequences are distant from each other and degenerate primers are needed.
GeneFisher: Interactive Primer Design
Melting – this program computes, for a nucleic acid duplex, the enthalpy, the entropy and the melting temperature of the helix-coil transitions.
OLIGO 6, Primer Analysis Software for Mac and Windows.
OLIGO 6, Primer Analysis Software for Mac and Windows.
PCR primer selection program based on xprimer.
Primer Designer 4 for Windows 95 — Software to design, analyze, mutate primers.
Primer Premier: Comprehensive primer design for Windows and Power Macintosh.
Primer3 – PCR primers from nucleotide sequence
Primerfinder – A tool to design oligonucleotides suitable for PCR within any sequence you specify. Primers are designed to meet your specific needs based on the information entered on the search page.
Prodoric – prokaryotic database of gene-regulation and molecular networks.
You can easily design primers for PCR and DNA sequencing using our free Web DoPrimer software ! The sequence information you need can be retrieved on-the-fly from the EMBL sequence database.

1 Comment

  1. said,

    March 1, 2012 @ 12:51 am

    Thanks for publishing quality work on PCR Assays and
    PCR Primers. It is very uncommon to find useful resources on PCR nowadays.

RSS comments feed